For the determination of human chorionic gonadrotropic (hCG) in human urine. As the test sample diffuses through the reaction pack, the labeled antibody-dye conjugate binds to the hCG in the specimen. The complex formed binds to the anti-hCG antibody in the positive reaction zone and produces a pink color band when hCG concentration is ≥25 mIU/mL. In the absence of hCG, there is no line in the positive reaction zone. Unbound conjugate binds to the reagents in the negative control zone, producing a pink color band, demonstrating that the reagents and reaction pack are functioning correctly.